Cell-based, bioluminescent assay for monitoring the interaction between PCSK9 and the LDL receptor

Monitoring the expression of cell-surface receptors, their interaction with extracellular ligands, and their fate upon ligand binding is important for understanding receptor function and developing new therapies. We describe a cell-based method that utilizes bioluminescent protein complementation te...

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Bibliographic Details
Main Authors: Sarah J. Duellman, Thomas Machleidt, James J. Cali, Jolanta Vidugiriene
Format: Article
Language:English
Published: Elsevier 2017-08-01
Series:Journal of Lipid Research
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520337974