Plant Chromosome-Specific Probes by Microdissection of a Single Chromosome: Is That a Reality?

Plant Chromosome-Specific Probes by Microdissection of a Single Chromosome: Is That a Reality?

Painting plant chromosomes through chromosomal in situ suppression (CISS) hybridization has long been considered impracticable. Seeking to build specific and complex probes from a single microdissected chromosome, we employed human chromosomes as models to standardize all the necessary steps for app...

Full description

Bibliographic Details
Main Authors: Fernanda Aparecida Ferrari Soares, Carlos Roberto Carvalho, Mariana Cansian Sattler, Jéssica Coutinho Silva, Denise Eliane Euzébio Pinto, Paulo Zanchetta Passamani, Alex Junior Silva, Wellington Ronildo Clarindo
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-03-01
Series:Frontiers in Plant Science
Subjects:
molecular cytogenetics
chromosome painting
micromanipulation
DOP-PCR
maize
pepper
Online Access:https://www.frontiersin.org/article/10.3389/fpls.2020.00334/full

Internet

https://www.frontiersin.org/article/10.3389/fpls.2020.00334/full

Similar Items