Effect of calcium on porcine ICSI embryos expressing EGFP is related to activation of ooplasmic DNase I
Several reliable methods to produce transgenic animals use the male genome. After penetration into oocytes, sperm DNA undergoes dramatic conformational changes that might represent an opportunity for exogenous DNA to integrate into the zygote genome. A nuclease, DNase I, with Ca<sup>2+</sup...
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| Format: | Article |
| Language: | English |
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Higher Education Press
2015-03-01
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| Series: | Frontiers of Agricultural Science and Engineering |
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| Online Access: | http://academic.hep.com.cn/fase/fileup/2095-7505/PDF/2095-7505-2015-1-84.pdf |