EARLY gestational exposure to isoflurane causes persistent cell loss in the dentate gyrus of adult male rats
Abstract Background Our previous research showed that 4 h of maternal anesthesia with isoflurane during early gestation in pregnant rats leads to a deficit in spatial memory of adult male offspring. Because spatial memory is predominantly a hippocampally-mediated task, we asked the question if early...
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doaj-5b5e7fc977ac441a8aaaa791c12ced312020-11-25T01:04:20ZengBMCBehavioral and Brain Functions1744-90812017-12-011311510.1186/s12993-017-0132-5EARLY gestational exposure to isoflurane causes persistent cell loss in the dentate gyrus of adult male ratsArvind Palanisamy0Gregory Crosby1Deborah J. Culley2Department of Anesthesiology, Perioperative and Pain Medicine, Brigham and Women’s Hospital, Harvard Medical SchoolDepartment of Anesthesiology, Perioperative and Pain Medicine, Brigham and Women’s Hospital, Harvard Medical SchoolDepartment of Anesthesiology, Perioperative and Pain Medicine, Brigham and Women’s Hospital, Harvard Medical SchoolAbstract Background Our previous research showed that 4 h of maternal anesthesia with isoflurane during early gestation in pregnant rats leads to a deficit in spatial memory of adult male offspring. Because spatial memory is predominantly a hippocampally-mediated task, we asked the question if early gestational exposure to isoflurane affects development of the hippocampus in the offspring. Findings Previously behaviorally characterized adult male rats that were exposed to isoflurane during second trimester were sacrificed at 4 months of age (N = 10 and 13, control and isoflurane groups, respectively) for quantitative histology of hippocampal subregions. Sections were stained with cresyl violet and the total number of cells in the granular layer of the dentate gyrus and the pyramidal cell layer in the CA1 region were determined by a blinded observer using unbiased stereological principles and the optical fractionator method. Data were analyzed using Student’s t test; P < 0.05 was accorded statistical significance. Stereological examination revealed 9% fewer cells in the granular layer of the dentate gyrus of isoflurane-exposed adult rats compared to controls (1,002,122 ± 84,870 vs. 1,091,829 ± 65,791, respectively; Mean ± S.D, *P = 0.01). In contrast, there were no changes in the cell number in the CA1 region, nor were there changes in the volumes of both regions. Conclusions Our results show that maternal isoflurane anesthesia in rodents causes region-specific cell loss in the hippocampus of adult male offspring. These changes may, in part, account for the behavioral deficits reported in adult rats exposed to isoflurane in utero.http://link.springer.com/article/10.1186/s12993-017-0132-5Anesthesia during pregnancyAnesthetic neurotoxicityMaternal anesthesiaSpatial working memoryStereologyHippocampus |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Arvind Palanisamy Gregory Crosby Deborah J. Culley |
spellingShingle |
Arvind Palanisamy Gregory Crosby Deborah J. Culley EARLY gestational exposure to isoflurane causes persistent cell loss in the dentate gyrus of adult male rats Behavioral and Brain Functions Anesthesia during pregnancy Anesthetic neurotoxicity Maternal anesthesia Spatial working memory Stereology Hippocampus |
author_facet |
Arvind Palanisamy Gregory Crosby Deborah J. Culley |
author_sort |
Arvind Palanisamy |
title |
EARLY gestational exposure to isoflurane causes persistent cell loss in the dentate gyrus of adult male rats |
title_short |
EARLY gestational exposure to isoflurane causes persistent cell loss in the dentate gyrus of adult male rats |
title_full |
EARLY gestational exposure to isoflurane causes persistent cell loss in the dentate gyrus of adult male rats |
title_fullStr |
EARLY gestational exposure to isoflurane causes persistent cell loss in the dentate gyrus of adult male rats |
title_full_unstemmed |
EARLY gestational exposure to isoflurane causes persistent cell loss in the dentate gyrus of adult male rats |
title_sort |
early gestational exposure to isoflurane causes persistent cell loss in the dentate gyrus of adult male rats |
publisher |
BMC |
series |
Behavioral and Brain Functions |
issn |
1744-9081 |
publishDate |
2017-12-01 |
description |
Abstract Background Our previous research showed that 4 h of maternal anesthesia with isoflurane during early gestation in pregnant rats leads to a deficit in spatial memory of adult male offspring. Because spatial memory is predominantly a hippocampally-mediated task, we asked the question if early gestational exposure to isoflurane affects development of the hippocampus in the offspring. Findings Previously behaviorally characterized adult male rats that were exposed to isoflurane during second trimester were sacrificed at 4 months of age (N = 10 and 13, control and isoflurane groups, respectively) for quantitative histology of hippocampal subregions. Sections were stained with cresyl violet and the total number of cells in the granular layer of the dentate gyrus and the pyramidal cell layer in the CA1 region were determined by a blinded observer using unbiased stereological principles and the optical fractionator method. Data were analyzed using Student’s t test; P < 0.05 was accorded statistical significance. Stereological examination revealed 9% fewer cells in the granular layer of the dentate gyrus of isoflurane-exposed adult rats compared to controls (1,002,122 ± 84,870 vs. 1,091,829 ± 65,791, respectively; Mean ± S.D, *P = 0.01). In contrast, there were no changes in the cell number in the CA1 region, nor were there changes in the volumes of both regions. Conclusions Our results show that maternal isoflurane anesthesia in rodents causes region-specific cell loss in the hippocampus of adult male offspring. These changes may, in part, account for the behavioral deficits reported in adult rats exposed to isoflurane in utero. |
topic |
Anesthesia during pregnancy Anesthetic neurotoxicity Maternal anesthesia Spatial working memory Stereology Hippocampus |
url |
http://link.springer.com/article/10.1186/s12993-017-0132-5 |
work_keys_str_mv |
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