Construction and characterization of a novel glucose dehydrogenase-leucine dehydrogenase fusion enzyme for the biosynthesis of l-tert-leucine

Construction and characterization of a novel glucose dehydrogenase-leucine dehydrogenase fusion enzyme for the biosynthesis of l-tert-leucine

Abstract Background Biosynthesis of l-tert-leucine (l-tle), a significant pharmaceutical intermediate, by a cofactor regeneration system friendly and efficiently is a worthful goal all the time. The cofactor regeneration system of leucine dehydrogenase (LeuDH) and glucose dehydrogenase (GDH) has sho...

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Bibliographic Details
Main Authors: Langxing Liao, Yonghui Zhang, Yali Wang, Yousi Fu, Aihui Zhang, Ruodian Qiu, Shuhao Yang, Baishan Fang
Format: Article
Language:English
Published: BMC 2021-01-01
Series:Microbial Cell Factories
Subjects:
l-tert-leucine
Glucose dehydrogenase
Leucine dehydrogenase
Fusion enzyme
Whole cells
Online Access:https://doi.org/10.1186/s12934-020-01501-2

Internet

https://doi.org/10.1186/s12934-020-01501-2

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