Construction and characterization of a novel glucose dehydrogenase-leucine dehydrogenase fusion enzyme for the biosynthesis of l-tert-leucine
Abstract Background Biosynthesis of l-tert-leucine (l-tle), a significant pharmaceutical intermediate, by a cofactor regeneration system friendly and efficiently is a worthful goal all the time. The cofactor regeneration system of leucine dehydrogenase (LeuDH) and glucose dehydrogenase (GDH) has sho...
Main Authors: | Langxing Liao, Yonghui Zhang, Yali Wang, Yousi Fu, Aihui Zhang, Ruodian Qiu, Shuhao Yang, Baishan Fang |
---|---|
Format: | Article |
Language: | English |
Published: |
BMC
2021-01-01
|
Series: | Microbial Cell Factories |
Subjects: | |
Online Access: | https://doi.org/10.1186/s12934-020-01501-2 |
Similar Items
-
Cloning and Expression of a Novel Leucine Dehydrogenase: Characterization and L-tert-Leucine Production
by: Wei Luo, et al.
Published: (2020-03-01) -
Biosynthetic L-tert-leucine using Escherichia coli co-expressing a novel NADH-dependent leucine dehydrogenase and a formate dehydrogenase
by: Longxing Wang, et al.
Published: (2020-09-01) -
Expression of Novel L-Leucine Dehydrogenase and High-Level Production of L-Tert-Leucine Catalyzed by Engineered Escherichia coli
by: Yuan-Yuan Jia, et al.
Published: (2021-03-01) -
Peptide Bond Formation Between the Hetrosubunits of ω-Transaminase, Alanine Dehydrogenase, and Formate Dehydrogenase Through Subunit Splicing Promoted by Heterodimerization of Leucine Zipper Motifs
by: Rong Li, et al.
Published: (2020-06-01) -
A Novel Cold-Adapted Leucine Dehydrogenase from Antarctic Sea-Ice Bacterium Pseudoalteromonas sp. ANT178
by: Yatong Wang, et al.
Published: (2018-10-01)