Targeted capture-based NGS is superior to multiplex PCR-based NGS for hereditary BRCA1 and BRCA2 gene analysis in FFPE tumor samples

Targeted capture-based NGS is superior to multiplex PCR-based NGS for hereditary BRCA1 and BRCA2 gene analysis in FFPE tumor samples

Abstract Background With the introduction of Olaparib treatment for BRCA-deficient recurrent ovarian cancer, testing for somatic and/or germline mutations in BRCA1/2 genes in tumor tissues became essential for treatment decisions. In most cases only formalin-fixed paraffin-embedded (FFPE) samples, c...

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Bibliographic Details
Main Authors: Falk Zakrzewski, Laura Gieldon, Andreas Rump, Michael Seifert, Konrad Grützmann, Alexander Krüger, Sina Loos, Silke Zeugner, Karl Hackmann, Joseph Porrmann, Johannes Wagner, Karin Kast, Pauline Wimberger, Gustavo Baretton, Evelin Schröck, Daniela Aust, Barbara Klink
Format: Article
Language:English
Published: BMC 2019-04-01
Series:BMC Cancer
Subjects:
HBOC
Genetic testing
Pathogenic germline mutations
NGS
BRCA1
BRCA2
Online Access:http://link.springer.com/article/10.1186/s12885-019-5584-6

Internet

http://link.springer.com/article/10.1186/s12885-019-5584-6

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