CUTseq is a versatile method for preparing multiplexed DNA sequencing libraries from low-input samples

CUTseq is a versatile method for preparing multiplexed DNA sequencing libraries from low-input samples

Genomics DNA library preparation from formalin-fixed paraffin-embedded tissues is challenging. Here the authors describe CUTseq that uses restriction enzymes and in vitro amplification to barcode samples for reduced representation genome sequencing.

Bibliographic Details
Main Authors: Xiaolu Zhang, Silvano Garnerone, Michele Simonetti, Luuk Harbers, Marcin Nicoś, Reza Mirzazadeh, Tiziana Venesio, Anna Sapino, Johan Hartman, Caterina Marchiò, Magda Bienko, Nicola Crosetto
Format: Article
Language:English
Published: Nature Publishing Group 2019-10-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-019-12570-2

Internet

https://doi.org/10.1038/s41467-019-12570-2

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