CUTseq is a versatile method for preparing multiplexed DNA sequencing libraries from low-input samples

CUTseq is a versatile method for preparing multiplexed DNA sequencing libraries from low-input samples

Genomics DNA library preparation from formalin-fixed paraffin-embedded tissues is challenging. Here the authors describe CUTseq that uses restriction enzymes and in vitro amplification to barcode samples for reduced representation genome sequencing.

Bibliographic Details
Main Authors: Xiaolu Zhang, Silvano Garnerone, Michele Simonetti, Luuk Harbers, Marcin Nicoś, Reza Mirzazadeh, Tiziana Venesio, Anna Sapino, Johan Hartman, Caterina Marchiò, Magda Bienko, Nicola Crosetto
Format: Article
Language:English
Published: Nature Publishing Group 2019-10-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-019-12570-2
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spelling doaj-5bd13737717a4dc7914df1406868bf3e2021-05-11T11:51:29ZengNature Publishing GroupNature Communications2041-17232019-10-0110111210.1038/s41467-019-12570-2CUTseq is a versatile method for preparing multiplexed DNA sequencing libraries from low-input samplesXiaolu Zhang0Silvano Garnerone1Michele Simonetti2Luuk Harbers3Marcin Nicoś4Reza Mirzazadeh5Tiziana Venesio6Anna Sapino7Johan Hartman8Caterina Marchiò9Magda Bienko10Nicola Crosetto11Science for Life Laboratory, Department of Medical Biochemistry and Biophysics, Karolinska InstitutetScience for Life Laboratory, Department of Medical Biochemistry and Biophysics, Karolinska InstitutetScience for Life Laboratory, Department of Medical Biochemistry and Biophysics, Karolinska InstitutetScience for Life Laboratory, Department of Medical Biochemistry and Biophysics, Karolinska InstitutetScience for Life Laboratory, Department of Medical Biochemistry and Biophysics, Karolinska InstitutetScience for Life Laboratory, Department of Medical Biochemistry and Biophysics, Karolinska InstitutetPathology Unit, Candiolo Cancer Institute, FPO-IRCCSPathology Unit, Candiolo Cancer Institute, FPO-IRCCSDepartment of Oncology and Pathology, Karolinska InstitutetPathology Unit, Candiolo Cancer Institute, FPO-IRCCSScience for Life Laboratory, Department of Medical Biochemistry and Biophysics, Karolinska InstitutetScience for Life Laboratory, Department of Medical Biochemistry and Biophysics, Karolinska InstitutetGenomics DNA library preparation from formalin-fixed paraffin-embedded tissues is challenging. Here the authors describe CUTseq that uses restriction enzymes and in vitro amplification to barcode samples for reduced representation genome sequencing.https://doi.org/10.1038/s41467-019-12570-2
collection DOAJ
language English
format Article
sources DOAJ
author Xiaolu Zhang
Silvano Garnerone
Michele Simonetti
Luuk Harbers
Marcin Nicoś
Reza Mirzazadeh
Tiziana Venesio
Anna Sapino
Johan Hartman
Caterina Marchiò
Magda Bienko
Nicola Crosetto
spellingShingle Xiaolu Zhang
Silvano Garnerone
Michele Simonetti
Luuk Harbers
Marcin Nicoś
Reza Mirzazadeh
Tiziana Venesio
Anna Sapino
Johan Hartman
Caterina Marchiò
Magda Bienko
Nicola Crosetto
CUTseq is a versatile method for preparing multiplexed DNA sequencing libraries from low-input samples
Nature Communications
author_facet Xiaolu Zhang
Silvano Garnerone
Michele Simonetti
Luuk Harbers
Marcin Nicoś
Reza Mirzazadeh
Tiziana Venesio
Anna Sapino
Johan Hartman
Caterina Marchiò
Magda Bienko
Nicola Crosetto
author_sort Xiaolu Zhang
title CUTseq is a versatile method for preparing multiplexed DNA sequencing libraries from low-input samples
title_short CUTseq is a versatile method for preparing multiplexed DNA sequencing libraries from low-input samples
title_full CUTseq is a versatile method for preparing multiplexed DNA sequencing libraries from low-input samples
title_fullStr CUTseq is a versatile method for preparing multiplexed DNA sequencing libraries from low-input samples
title_full_unstemmed CUTseq is a versatile method for preparing multiplexed DNA sequencing libraries from low-input samples
title_sort cutseq is a versatile method for preparing multiplexed dna sequencing libraries from low-input samples
publisher Nature Publishing Group
series Nature Communications
issn 2041-1723
publishDate 2019-10-01
description Genomics DNA library preparation from formalin-fixed paraffin-embedded tissues is challenging. Here the authors describe CUTseq that uses restriction enzymes and in vitro amplification to barcode samples for reduced representation genome sequencing.
url https://doi.org/10.1038/s41467-019-12570-2
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