Single-Step Incubation Determination of miRNAs in Cancer Cells Using an Amperometric Biosensor Based on Competitive Hybridization onto Magnetic Beads

Single-Step Incubation Determination of miRNAs in Cancer Cells Using an Amperometric Biosensor Based on Competitive Hybridization onto Magnetic Beads

This work reports an amperometric biosensor for the determination of miRNA-21, a relevant oncogene. The methodology involves a competitive DNA-target miRNA hybridization assay performed on the surface of magnetic microbeads (MBs) and amperometric transduction at screen-printed carbon electrodes (SPC...

Full description

Bibliographic Details
Main Authors: Eva Vargas, Eloy Povedano, Víctor Ruiz-Valdepeñas Montiel, Rebeca M. Torrente-Rodríguez, Mohamed Zouari, Juan José Montoya, Noureddine Raouafi, Susana Campuzano, José M. Pingarrón
Format: Article
Language:English
Published: MDPI AG 2018-03-01
Series:Sensors
Subjects:
miRNA
screen-printed electrode
amperometry
competitive assay
magnetic beads
cancer cells
Online Access:http://www.mdpi.com/1424-8220/18/3/863

Internet

http://www.mdpi.com/1424-8220/18/3/863

Similar Items