TNF-alpha neutralizing antibody blocks thermal sensitivity induced by compound 48/80-provoked mast cell degranulation [v1; ref status: indexed, http://f1000r.es/1mq]
Background: Neuro-inflammatory circuits in the tissue regulate the complex pathophysiology of pain. Protective nociceptive pain serves as an early warning system against noxious environmental stimuli. Tissue-resident mast cells orchestrate the increased thermal sensitivity following injection of...
Main Authors: | , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
F1000 Research Ltd
2013-08-01
|
Series: | F1000Research |
Subjects: | |
Online Access: | http://f1000research.com/articles/2-178/v1 |
id |
doaj-5c15661c7412450cab03cc09cf9c721f |
---|---|
record_format |
Article |
spelling |
doaj-5c15661c7412450cab03cc09cf9c721f2020-11-25T01:24:06ZengF1000 Research LtdF1000Research2046-14022013-08-01210.12688/f1000research.2-178.v12114TNF-alpha neutralizing antibody blocks thermal sensitivity induced by compound 48/80-provoked mast cell degranulation [v1; ref status: indexed, http://f1000r.es/1mq]Devavani Chatterjea0Luisa Paredes1Tijana Martinov2Evelyn Balsells3Juliann Allen4Akilah Sykes5Alyssa Ashbaugh6Biology Department, Macalester College, St. Paul, MN 55015, USABiology Department, Macalester College, St. Paul, MN 55015, USABiology Department, Macalester College, St. Paul, MN 55015, USABiology Department, Macalester College, St. Paul, MN 55015, USABiology Department, Macalester College, St. Paul, MN 55015, USABiology Department, Macalester College, St. Paul, MN 55015, USABiology Department, Macalester College, St. Paul, MN 55015, USABackground: Neuro-inflammatory circuits in the tissue regulate the complex pathophysiology of pain. Protective nociceptive pain serves as an early warning system against noxious environmental stimuli. Tissue-resident mast cells orchestrate the increased thermal sensitivity following injection of basic secretagogue compound 48/80 in the hind paw tissues of ND4 mice. Here we investigated the effects of pre-treatment with TNF-α neutralizing antibody on compound 48/80-provoked thermal hyperalgesia. Methods: We treated ND4 Swiss male mice with intravenous anti-TNF-α antibody or vehicle 30 minutes prior to bilateral, intra-plantar compound 48/80 administration and measured changes in the timing of hind paw withdrawal observed subsequent to mice being placed on a 51oC hotplate. We also assessed changes in tissue swelling, TNF-α gene expression and protein abundance, mast cell degranulation, and neutrophil influx in the hind paw tissue. Findings: We found that TNF-α neutralization significantly blocked thermal hyperalgesia, and reduced early tissue swelling. TNF-α neutralization had no significant effect on mast cell degranulation or neutrophil influx into the tissue, however. Moreover, no changes in TNF-α protein or mRNA levels were detected within 3 hours of administration of compound 48/80. Interpretation: The neutralizing antibodies likely target pre-formed TNF-α including that stored in the granules of tissue-resident mast cells. Pre-formed TNF-α, released upon degranulation, has immediate effects on nociceptive signaling prior to the induction of neutrophil influx. These direct effects on nociceptors are abrogated by TNF-α blockade resulting in compromised nociceptive withdrawal responses to acute, harmful environmental stimuli.http://f1000research.com/articles/2-178/v1Allergy & HypersensitivityLeukocyte Signaling & Gene Expression |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Devavani Chatterjea Luisa Paredes Tijana Martinov Evelyn Balsells Juliann Allen Akilah Sykes Alyssa Ashbaugh |
spellingShingle |
Devavani Chatterjea Luisa Paredes Tijana Martinov Evelyn Balsells Juliann Allen Akilah Sykes Alyssa Ashbaugh TNF-alpha neutralizing antibody blocks thermal sensitivity induced by compound 48/80-provoked mast cell degranulation [v1; ref status: indexed, http://f1000r.es/1mq] F1000Research Allergy & Hypersensitivity Leukocyte Signaling & Gene Expression |
author_facet |
Devavani Chatterjea Luisa Paredes Tijana Martinov Evelyn Balsells Juliann Allen Akilah Sykes Alyssa Ashbaugh |
author_sort |
Devavani Chatterjea |
title |
TNF-alpha neutralizing antibody blocks thermal sensitivity induced by compound 48/80-provoked mast cell degranulation [v1; ref status: indexed, http://f1000r.es/1mq] |
title_short |
TNF-alpha neutralizing antibody blocks thermal sensitivity induced by compound 48/80-provoked mast cell degranulation [v1; ref status: indexed, http://f1000r.es/1mq] |
title_full |
TNF-alpha neutralizing antibody blocks thermal sensitivity induced by compound 48/80-provoked mast cell degranulation [v1; ref status: indexed, http://f1000r.es/1mq] |
title_fullStr |
TNF-alpha neutralizing antibody blocks thermal sensitivity induced by compound 48/80-provoked mast cell degranulation [v1; ref status: indexed, http://f1000r.es/1mq] |
title_full_unstemmed |
TNF-alpha neutralizing antibody blocks thermal sensitivity induced by compound 48/80-provoked mast cell degranulation [v1; ref status: indexed, http://f1000r.es/1mq] |
title_sort |
tnf-alpha neutralizing antibody blocks thermal sensitivity induced by compound 48/80-provoked mast cell degranulation [v1; ref status: indexed, http://f1000r.es/1mq] |
publisher |
F1000 Research Ltd |
series |
F1000Research |
issn |
2046-1402 |
publishDate |
2013-08-01 |
description |
Background: Neuro-inflammatory circuits in the tissue regulate the complex pathophysiology of pain. Protective nociceptive pain serves as an early warning system against noxious environmental stimuli. Tissue-resident mast cells orchestrate the increased thermal sensitivity following injection of basic secretagogue compound 48/80 in the hind paw tissues of ND4 mice. Here we investigated the effects of pre-treatment with TNF-α neutralizing antibody on compound 48/80-provoked thermal hyperalgesia. Methods: We treated ND4 Swiss male mice with intravenous anti-TNF-α antibody or vehicle 30 minutes prior to bilateral, intra-plantar compound 48/80 administration and measured changes in the timing of hind paw withdrawal observed subsequent to mice being placed on a 51oC hotplate. We also assessed changes in tissue swelling, TNF-α gene expression and protein abundance, mast cell degranulation, and neutrophil influx in the hind paw tissue. Findings: We found that TNF-α neutralization significantly blocked thermal hyperalgesia, and reduced early tissue swelling. TNF-α neutralization had no significant effect on mast cell degranulation or neutrophil influx into the tissue, however. Moreover, no changes in TNF-α protein or mRNA levels were detected within 3 hours of administration of compound 48/80. Interpretation: The neutralizing antibodies likely target pre-formed TNF-α including that stored in the granules of tissue-resident mast cells. Pre-formed TNF-α, released upon degranulation, has immediate effects on nociceptive signaling prior to the induction of neutrophil influx. These direct effects on nociceptors are abrogated by TNF-α blockade resulting in compromised nociceptive withdrawal responses to acute, harmful environmental stimuli. |
topic |
Allergy & Hypersensitivity Leukocyte Signaling & Gene Expression |
url |
http://f1000research.com/articles/2-178/v1 |
work_keys_str_mv |
AT devavanichatterjea tnfalphaneutralizingantibodyblocksthermalsensitivityinducedbycompound4880provokedmastcelldegranulationv1refstatusindexedhttpf1000res1mq AT luisaparedes tnfalphaneutralizingantibodyblocksthermalsensitivityinducedbycompound4880provokedmastcelldegranulationv1refstatusindexedhttpf1000res1mq AT tijanamartinov tnfalphaneutralizingantibodyblocksthermalsensitivityinducedbycompound4880provokedmastcelldegranulationv1refstatusindexedhttpf1000res1mq AT evelynbalsells tnfalphaneutralizingantibodyblocksthermalsensitivityinducedbycompound4880provokedmastcelldegranulationv1refstatusindexedhttpf1000res1mq AT juliannallen tnfalphaneutralizingantibodyblocksthermalsensitivityinducedbycompound4880provokedmastcelldegranulationv1refstatusindexedhttpf1000res1mq AT akilahsykes tnfalphaneutralizingantibodyblocksthermalsensitivityinducedbycompound4880provokedmastcelldegranulationv1refstatusindexedhttpf1000res1mq AT alyssaashbaugh tnfalphaneutralizingantibodyblocksthermalsensitivityinducedbycompound4880provokedmastcelldegranulationv1refstatusindexedhttpf1000res1mq |
_version_ |
1725118813150642176 |