Fluorescence Lifetime and Intensity of Thioflavin T as Reporters of Different Fibrillation Stages: Insights Obtained from Fluorescence Up-Conversion and Particle Size Distribution Measurements

Fluorescence Lifetime and Intensity of Thioflavin T as Reporters of Different Fibrillation Stages: Insights Obtained from Fluorescence Up-Conversion and Particle Size Distribution Measurements

Thioflavin T (ThT) assay is extensively used for studying fibrillation kinetics in vitro. However, the differences in the time course of ThT fluorescence intensity and lifetime and other physical parameters of the system, such as particle size distribution, raise questions about the correct interpre...

Full description

Bibliographic Details
Main Authors: Nataliya R. Rovnyagina, Gleb S. Budylin, Yuri G. Vainer, Tatiana N. Tikhonova, Sergey L. Vasin, Alexander A. Yakovlev, Victor O. Kompanets, Sergey V. Chekalin, Alexander V. Priezzhev, Evgeny A. Shirshin
Format: Article
Language:English
Published: MDPI AG 2020-08-01
Series:International Journal of Molecular Sciences
Subjects:
time-correlated single photon counting (TCSPC)
fluorescence up-conversion
dynamic light scattering (DLS)
nanoparticle tracking analysis (NTA)
fibrils
thioflavin T (ThT)
Online Access:https://www.mdpi.com/1422-0067/21/17/6169

Internet

https://www.mdpi.com/1422-0067/21/17/6169

Similar Items