Evaluation of Rate of Adhesion of <i>Lactobacillus namurensis</i> Strain GYP-74 to Porous Fine Ceramics

• Main Authors: Yousuke Taoka, Kentaro Sakai, Hiroyuki Kinoshita, Hanako Fukuyama, Taichi Kobayashi, Atsushi Hirano, Noriaki Kuroki, Michiyo Kuroki, Akihiko Kimura
• Format: Article
• Language: English
• Published: MDPI AG 2021-04-01
• Series: Processes
• Subjects: lactic acid bacteria; adherence; polyacetal; pore distribution; porous fine ceramics; probiotics
• Online Access: https://www.mdpi.com/2227-9717/9/4/658

This study aimed to evaluate the ratio of adherence of lactic acid bacteria (LAB) to porous fine ceramics in order to develop a novel LAB-rich pickle container for the production of functional fermented vegetables. Some LAB were isolated from the salted rice bran used for pickling (Nukadoko in Japanese). These isolates were classified in <i>Lactobacillus namurensis</i> by phylogenetic analysis. Some pottery-shard (PS) samples were prepared by varying the mixing rate of polyacetal (POM) resin to clay (0–30% (<i>v/v</i>)) and the burning temperature (1000 °C or 1100 °C). A test of the adherence of strain GYP-74 to the PSs was performed. The results showed that the adherence rate was significantly higher in the PSs burned at 1100 °C as compared with those burned at 1000 °C. A pore distribution analysis showed that pore sizes of less than a few µm and pore sizes in the range of a few µm to a few hundred µm were mainly distributed in the PSs without and with POM, respectively. X-ray diffraction analysis showed that both PSs with and without POM contained quartz and hematite. The PSs burned at 1000 °C and 1100 °C specifically contained microcline and mullite, respectively. This study revealed the basal information regarding what makes PSs adequate for LAB adhesion.