Use of brief culture on Lowenstein–Jensen media as pre-polymerase chain reaction (Pre-PCR) to detect Mycobacterium

Use of brief culture on Lowenstein–Jensen media as pre-polymerase chain reaction (Pre-PCR) to detect Mycobacterium

Background and aim: Mycobacterium tuberculosis (MTB), the causative agent of tuberculosis (TB), does not retain any bacteriological stain due to the high lipid content in its wall. Classic methods like acid-fast staining or Ziehl–Neelsen staining are used for the diagnosis. Nucleic acid amplificatio...

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Bibliographic Details
Main Authors: Fatemeh Fallah, Abdollah Karimi, Gita Eslami, Bakhtiar Yousefi, Bahador Yazdanpanah, Arezou Taherpour, Mozhgan Esmaeili
Format: Article
Language:English
Published: Wolters Kluwer Medknow Publications 2015-01-01
Series:International Journal of Mycobacteriology
Subjects:
Mycobacteria
Tuberculosis
Polymerase chain reaction (PCR)
Brief-culture
Online Access:http://www.ijmyco.org/article.asp?issn=2212-5531;year=2015;volume=4;issue=5;spage=90;epage=90;aulast=Fallah;type=0

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http://www.ijmyco.org/article.asp?issn=2212-5531;year=2015;volume=4;issue=5;spage=90;epage=90;aulast=Fallah;type=0

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