Effect of a polyphenol-rich dietary pattern on intestinal permeability and gut and blood microbiomics in older subjects: study protocol of the MaPLE randomised controlled trial
Abstract Background During aging, alterations of the intestinal microbial ecosystem can occur contributing to immunosenescence, inflamm-aging and impairment of intestinal barrier function (increased intestinal permeability; IP). In the context of a diet-microbiota-IP axis in older subjects, food bio...
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BMC
2020-02-01
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Series: | BMC Geriatrics |
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Online Access: | http://link.springer.com/article/10.1186/s12877-020-1472-9 |
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Article |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Simone Guglielmetti Stefano Bernardi Cristian Del Bo’ Antonio Cherubini Marisa Porrini Giorgio Gargari Nicole Hidalgo-Liberona Raul Gonzalez-Dominguez Gregorio Peron Raul Zamora-Ros Mark S. Winterbone Benjamin Kirkup Paul A. Kroon Cristina Andres-Lacueva Patrizia Riso |
spellingShingle |
Simone Guglielmetti Stefano Bernardi Cristian Del Bo’ Antonio Cherubini Marisa Porrini Giorgio Gargari Nicole Hidalgo-Liberona Raul Gonzalez-Dominguez Gregorio Peron Raul Zamora-Ros Mark S. Winterbone Benjamin Kirkup Paul A. Kroon Cristina Andres-Lacueva Patrizia Riso Effect of a polyphenol-rich dietary pattern on intestinal permeability and gut and blood microbiomics in older subjects: study protocol of the MaPLE randomised controlled trial BMC Geriatrics Gut barrier function Leaky gut Flavonoids Phenolics Inflammation Aging |
author_facet |
Simone Guglielmetti Stefano Bernardi Cristian Del Bo’ Antonio Cherubini Marisa Porrini Giorgio Gargari Nicole Hidalgo-Liberona Raul Gonzalez-Dominguez Gregorio Peron Raul Zamora-Ros Mark S. Winterbone Benjamin Kirkup Paul A. Kroon Cristina Andres-Lacueva Patrizia Riso |
author_sort |
Simone Guglielmetti |
title |
Effect of a polyphenol-rich dietary pattern on intestinal permeability and gut and blood microbiomics in older subjects: study protocol of the MaPLE randomised controlled trial |
title_short |
Effect of a polyphenol-rich dietary pattern on intestinal permeability and gut and blood microbiomics in older subjects: study protocol of the MaPLE randomised controlled trial |
title_full |
Effect of a polyphenol-rich dietary pattern on intestinal permeability and gut and blood microbiomics in older subjects: study protocol of the MaPLE randomised controlled trial |
title_fullStr |
Effect of a polyphenol-rich dietary pattern on intestinal permeability and gut and blood microbiomics in older subjects: study protocol of the MaPLE randomised controlled trial |
title_full_unstemmed |
Effect of a polyphenol-rich dietary pattern on intestinal permeability and gut and blood microbiomics in older subjects: study protocol of the MaPLE randomised controlled trial |
title_sort |
effect of a polyphenol-rich dietary pattern on intestinal permeability and gut and blood microbiomics in older subjects: study protocol of the maple randomised controlled trial |
publisher |
BMC |
series |
BMC Geriatrics |
issn |
1471-2318 |
publishDate |
2020-02-01 |
description |
Abstract Background During aging, alterations of the intestinal microbial ecosystem can occur contributing to immunosenescence, inflamm-aging and impairment of intestinal barrier function (increased intestinal permeability; IP). In the context of a diet-microbiota-IP axis in older subjects, food bioactives such as polyphenols may play a beneficial modulatory role. Methods MaPLE is a project centered on a randomized, controlled cross-over dietary intervention trial [polyphenol-rich diet (PR-diet) versus control diet (C-diet)] targeted to older people (≥ 60 y) living in a well-controlled setting (i.e. nursing home). The 8-week interventions are separated by an 8-week wash-out period. Three small portions per day of selected polyphenol-rich foods are consumed during intervention in substitution of other comparable products within the C-diet. Biological samples are collected before and after each treatment period to evaluate markers related to IP, inflammation, vascular function, oxidative stress, gut and blood microbiomics, metabolomics. A sample size of 50 subjects was defined based on IP as primary outcome. Discussion Evidence that increasing the consumption of polyphenol-rich food products can positively affect intestinal microbial ecosystem resulting in reduced IP and decreased translocation of inflammogenic bacterial factors into the bloodstream will be provided. The integration of data from gut and blood microbiomics, metabolomics and other IP-related markers will improve the understanding of the beneficial effect of the intervention in the context of polyphenols−microbiota−IP interactions. Finally, findings obtained will provide a proof of concept of the reliability of the dietary intervention, also contributing to future implementations of dietary guidelines directed to IP management in the older and other at risk subjects. Trial registration The trial is registered at (ISRCTN10214981); April 28, 2017. |
topic |
Gut barrier function Leaky gut Flavonoids Phenolics Inflammation Aging |
url |
http://link.springer.com/article/10.1186/s12877-020-1472-9 |
work_keys_str_mv |
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doaj-5cb1e76b9ef541ef9ad0149dfa7d00922020-11-25T03:56:00ZengBMCBMC Geriatrics1471-23182020-02-0120111010.1186/s12877-020-1472-9Effect of a polyphenol-rich dietary pattern on intestinal permeability and gut and blood microbiomics in older subjects: study protocol of the MaPLE randomised controlled trialSimone Guglielmetti0Stefano Bernardi1Cristian Del Bo’2Antonio Cherubini3Marisa Porrini4Giorgio Gargari5Nicole Hidalgo-Liberona6Raul Gonzalez-Dominguez7Gregorio Peron8Raul Zamora-Ros9Mark S. Winterbone10Benjamin Kirkup11Paul A. Kroon12Cristina Andres-Lacueva13Patrizia Riso14Department of Food, Environmental and Nutritional Sciences (DeFENS), Università degli Studi di MilanoDepartment of Food, Environmental and Nutritional Sciences (DeFENS), Università degli Studi di MilanoDepartment of Food, Environmental and Nutritional Sciences (DeFENS), Università degli Studi di MilanoGeriatria, Accettazione Geriatrica e Centro di Ricerca per l’Invecchiamento, IRCCS INRCADepartment of Food, Environmental and Nutritional Sciences (DeFENS), Università degli Studi di MilanoDepartment of Food, Environmental and Nutritional Sciences (DeFENS), Università degli Studi di MilanoBiomarkers and Nutrimetabolomics Laboratory, Department of Nutrition, Food Sciences and Gastronomy, Food Technology Reference Net (XaRTA), Nutrition and Food Safety Research Institute (INSA), Faculty of Pharmacy and Food Sciences, University of BarcelonaBiomarkers and Nutrimetabolomics Laboratory, Department of Nutrition, Food Sciences and Gastronomy, Food Technology Reference Net (XaRTA), Nutrition and Food Safety Research Institute (INSA), Faculty of Pharmacy and Food Sciences, University of BarcelonaBiomarkers and Nutrimetabolomics Laboratory, Department of Nutrition, Food Sciences and Gastronomy, Food Technology Reference Net (XaRTA), Nutrition and Food Safety Research Institute (INSA), Faculty of Pharmacy and Food Sciences, University of BarcelonaBiomarkers and Nutrimetabolomics Laboratory, Department of Nutrition, Food Sciences and Gastronomy, Food Technology Reference Net (XaRTA), Nutrition and Food Safety Research Institute (INSA), Faculty of Pharmacy and Food Sciences, University of BarcelonaQuadram Institute Bioscience, Norwich Research ParkQuadram Institute Bioscience, Norwich Research ParkQuadram Institute Bioscience, Norwich Research ParkBiomarkers and Nutrimetabolomics Laboratory, Department of Nutrition, Food Sciences and Gastronomy, Food Technology Reference Net (XaRTA), Nutrition and Food Safety Research Institute (INSA), Faculty of Pharmacy and Food Sciences, University of BarcelonaDepartment of Food, Environmental and Nutritional Sciences (DeFENS), Università degli Studi di MilanoAbstract Background During aging, alterations of the intestinal microbial ecosystem can occur contributing to immunosenescence, inflamm-aging and impairment of intestinal barrier function (increased intestinal permeability; IP). In the context of a diet-microbiota-IP axis in older subjects, food bioactives such as polyphenols may play a beneficial modulatory role. Methods MaPLE is a project centered on a randomized, controlled cross-over dietary intervention trial [polyphenol-rich diet (PR-diet) versus control diet (C-diet)] targeted to older people (≥ 60 y) living in a well-controlled setting (i.e. nursing home). The 8-week interventions are separated by an 8-week wash-out period. Three small portions per day of selected polyphenol-rich foods are consumed during intervention in substitution of other comparable products within the C-diet. Biological samples are collected before and after each treatment period to evaluate markers related to IP, inflammation, vascular function, oxidative stress, gut and blood microbiomics, metabolomics. A sample size of 50 subjects was defined based on IP as primary outcome. Discussion Evidence that increasing the consumption of polyphenol-rich food products can positively affect intestinal microbial ecosystem resulting in reduced IP and decreased translocation of inflammogenic bacterial factors into the bloodstream will be provided. The integration of data from gut and blood microbiomics, metabolomics and other IP-related markers will improve the understanding of the beneficial effect of the intervention in the context of polyphenols−microbiota−IP interactions. Finally, findings obtained will provide a proof of concept of the reliability of the dietary intervention, also contributing to future implementations of dietary guidelines directed to IP management in the older and other at risk subjects. Trial registration The trial is registered at (ISRCTN10214981); April 28, 2017.http://link.springer.com/article/10.1186/s12877-020-1472-9Gut barrier functionLeaky gutFlavonoidsPhenolicsInflammationAging |