Combined FCS and PCH Analysis to Quantify Protein Dimerization in Living Cells

Combined FCS and PCH Analysis to Quantify Protein Dimerization in Living Cells

Protein dimerization plays a crucial role in the regulation of numerous biological processes. However, detecting protein dimers in a cellular environment is still a challenge. Here we present a methodology to measure the extent of dimerization of GFP-tagged proteins in living cells, using a combinat...

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Bibliographic Details
Main Authors: Laura M. Nederveen-Schippers, Pragya Pathak, Ineke Keizer-Gunnink, Adrie H. Westphal, Peter J. M. van Haastert, Jan Willem Borst, Arjan Kortholt, Victor Skakun
Format: Article
Language:English
Published: MDPI AG 2021-07-01
Series:International Journal of Molecular Sciences
Subjects:
brightness and diffusion global analysis
<i>Dictyostelium discoideum</i>
dimeric protein
GFP
FK506 binding protein 12
fluorescence correlation spectroscopy
Online Access:https://www.mdpi.com/1422-0067/22/14/7300

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https://www.mdpi.com/1422-0067/22/14/7300

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