Combined FCS and PCH Analysis to Quantify Protein Dimerization in Living Cells
Protein dimerization plays a crucial role in the regulation of numerous biological processes. However, detecting protein dimers in a cellular environment is still a challenge. Here we present a methodology to measure the extent of dimerization of GFP-tagged proteins in living cells, using a combinat...
Main Authors: | Laura M. Nederveen-Schippers, Pragya Pathak, Ineke Keizer-Gunnink, Adrie H. Westphal, Peter J. M. van Haastert, Jan Willem Borst, Arjan Kortholt, Victor Skakun |
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Format: | Article |
Language: | English |
Published: |
MDPI AG
2021-07-01
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Series: | International Journal of Molecular Sciences |
Subjects: | |
Online Access: | https://www.mdpi.com/1422-0067/22/14/7300 |
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