Single-Cell Isolation from Regenerating Murine Muscles for RNA-Sequencing Analysis

Summary: Single-cell RNA sequencing (scRNA-seq) is a powerful technique for deconvoluting and clustering thousands of otherwise intermingled cells based on their gene expression. Here, we present a complete protocol for the unbiased evaluation of regenerating murine skeletal muscle using scRNA-seq....

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Bibliographic Details
Main Authors: Stephanie N. Oprescu, Feng Yue, Shihuan Kuang
Format: Article
Language:English
Published: Elsevier 2020-09-01
Series:STAR Protocols
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166720300381