Enhanced expression of codon optimized Mycobacterium avium subsp. paratuberculosis antigens in Lactobacillus salivarius

Enhanced expression of codon optimized Mycobacterium avium subsp. paratuberculosis antigens in Lactobacillus salivarius

It is well documented that open reading frames containing high GC content show poor expression in A+T rich hosts. Specifically, G+C-rich codon usage is a limiting factor in heterologous expression of Mycobacterium avium subsp. paratuberculosis (MAP) proteins using Lactobacillus salivarius. However...

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Bibliographic Details
Main Authors: Christopher D Johnston, John P Bannantine, Rodney eGovender, Lorraine eEndersen, Daniel ePletzer, Helge eWeingart, Aidan eCoffey, Jim eO'Mahony, Roy D Sleator
Format: Article
Language:English
Published: Frontiers Media S.A. 2014-09-01
Series:Frontiers in Cellular and Infection Microbiology
Subjects:
Paratuberculosis
MMP
Johne's disease
codon optimization
MAP antigens
MptD
Online Access:http://journal.frontiersin.org/Journal/10.3389/fcimb.2014.00120/full

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http://journal.frontiersin.org/Journal/10.3389/fcimb.2014.00120/full

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