Enhanced expression of codon optimized Mycobacterium avium subsp. paratuberculosis antigens in Lactobacillus salivarius
It is well documented that open reading frames containing high GC content show poor expression in A+T rich hosts. Specifically, G+C-rich codon usage is a limiting factor in heterologous expression of Mycobacterium avium subsp. paratuberculosis (MAP) proteins using Lactobacillus salivarius. However...
Main Authors: | Christopher D Johnston, John P Bannantine, Rodney eGovender, Lorraine eEndersen, Daniel ePletzer, Helge eWeingart, Aidan eCoffey, Jim eO'Mahony, Roy D Sleator |
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Format: | Article |
Language: | English |
Published: |
Frontiers Media S.A.
2014-09-01
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Series: | Frontiers in Cellular and Infection Microbiology |
Subjects: | |
Online Access: | http://journal.frontiersin.org/Journal/10.3389/fcimb.2014.00120/full |
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