Identification of Mediator Kinase Substrates in Human Cells using Cortistatin A and Quantitative Phosphoproteomics

Identification of Mediator Kinase Substrates in Human Cells using Cortistatin A and Quantitative Phosphoproteomics

Cortistatin A (CA) is a highly selective inhibitor of the Mediator kinases CDK8 and CDK19. Using CA, we now report a large-scale identification of Mediator kinase substrates in human cells (HCT116). We identified over 16,000 quantified phosphosites including 78 high-confidence Mediator kinase target...

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Bibliographic Details
Main Authors: Zachary C. Poss, Christopher C. Ebmeier, Aaron T. Odell, Anupong Tangpeerachaikul, Thomas Lee, Henry E. Pelish, Matthew D. Shair, Robin D. Dowell, William M. Old, Dylan J. Taatjes
Format: Article
Language:English
Published: Elsevier 2016-04-01
Series:Cell Reports
Subjects:
CDK8-Mediator
Mediator
SILAC
cholesterol
MED13
MED13L
SIRT1
Online Access:http://www.sciencedirect.com/science/article/pii/S2211124716302868

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http://www.sciencedirect.com/science/article/pii/S2211124716302868

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