Efficient differentiation of human pluripotent stem cells into skeletal muscle cells by combining RNA-based MYOD1-expression and POU5F1-silencing

Efficient differentiation of human pluripotent stem cells into skeletal muscle cells by combining RNA-based MYOD1-expression and POU5F1-silencing

Abstract Direct generation of skeletal muscle cells from human pluripotent stem cells (hPSCs) would be beneficial for drug testing, drug discovery, and disease modelling in vitro. Here we show a rapid and robust method to induce myogenic differentiation of hPSCs by introducing mRNA encoding MYOD1 to...

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Bibliographic Details
Main Authors: Tomohiko Akiyama, Saeko Sato, Nana Chikazawa-Nohtomi, Atsumi Soma, Hiromi Kimura, Shunichi Wakabayashi, Shigeru B. H. Ko, Minoru S. H. Ko
Format: Article
Language:English
Published: Nature Publishing Group 2018-01-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-017-19114-y

Internet

https://doi.org/10.1038/s41598-017-19114-y

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