Quantification of Human Oral and Fecal Streptococcus parasanguinis by Use of Quantitative Real-Time PCR Targeting the groEL Gene

Quantification of Human Oral and Fecal Streptococcus parasanguinis by Use of Quantitative Real-Time PCR Targeting the groEL Gene

Two pairs of species-specific PCR primers targeting the housekeeping groEL gene, Spa146f-Spa525r and Spa93f-Spa525r, were designed to quantify human oral and fecal Streptococcus parasanguinis. Blast analysis against reference sequences of NCBI nucleotide collection database and the Chaperonin Sequen...

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Bibliographic Details
Main Authors: Qiurong Chen, Guojun Wu, Hui Chen, Hui Li, Shuo Li, Chenhong Zhang, Xiaoyan Pang, Linghua Wang, Liping Zhao, Jian Shen
Format: Article
Language:English
Published: Frontiers Media S.A. 2019-12-01
Series:Frontiers in Microbiology
Subjects:
Streptococcus parasanguinis
quantitative PCR
groEL gene
feces
saliva
Online Access:https://www.frontiersin.org/article/10.3389/fmicb.2019.02910/full

Internet

https://www.frontiersin.org/article/10.3389/fmicb.2019.02910/full

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