Isolation and purification of total RNA from Streptococcus mutans in suspension cultures and biofilms

The presence of extracellular polysaccharides matrix makes extraction and purification of RNA from Streptococcus mutans within biofilms challenging. In this study, several approaches to purify RNA extracted from S. mutans in suspension cultures and biofilms were examined. The combination of sonicati...

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Bibliographic Details
Main Authors: Jaime Aparecido Cury, Jennifer Seils, Hyun Koo
Format: Article
Language:English
Published: Sociedade Brasileira de Pesquisa Odontológica 2008-09-01
Series:Brazilian Oral Research
Subjects:
RNA
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242008000300005