Simultaneous Nucleic Acids Detection and Elimination of Carryover Contamination With Nanoparticles-Based Biosensor- and Antarctic Thermal Sensitive Uracil-DNA-Glycosylase-Supplemented Polymerase Spiral Reaction

Simultaneous Nucleic Acids Detection and Elimination of Carryover Contamination With Nanoparticles-Based Biosensor- and Antarctic Thermal Sensitive Uracil-DNA-Glycosylase-Supplemented Polymerase Spiral Reaction

The current report devised a novel isothermal diagnostic assay, termed as nanoparticle-based biosensor (NB)- and antarctic thermal sensitive uracil-DNA-glycosylase (ATSU)-supplemented polymerase spiral reaction (PSR; NB-ATSU-PSR). The technique merges enzymatic digestion of carryover contaminants an...

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Bibliographic Details
Main Authors: Yi Wang, Wei-wei Jiao, Yacui Wang, Lin Sun, Jie-qiong Li, Ze-ming Wang, Jing Xiao, Chen Shen, Fang Xu, Hui Qi, Yong-hong Wang, Ya-jie Guo, A-dong Shen
Format: Article
Language:English
Published: Frontiers Media S.A. 2019-12-01
Series:Frontiers in Bioengineering and Biotechnology
Subjects:
isothermal nucleic acid amplification
polymerase spiral reaction
nanoparticle-based biosensor
Klebsiella pneumoniae
carryover contamination
LoD
Online Access:https://www.frontiersin.org/article/10.3389/fbioe.2019.00401/full

Internet

https://www.frontiersin.org/article/10.3389/fbioe.2019.00401/full

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