Nearest-neighbor amino acids of specificity-determining residues influence the activity of engineered Cre-type recombinases
Abstract The tyrosine-type site-specific DNA recombinase Cre recombines its target site, loxP, with high activity and specificity without cross-recombining the target sites of highly related recombinases. Understanding how Cre achieves this precision is key to be able to rationally engineer site-spe...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Publishing Group
2020-08-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-020-70867-5 |