Implementation of an antibody characterization procedure and application to the major ALS/FTD disease gene C9ORF72

Implementation of an antibody characterization procedure and application to the major ALS/FTD disease gene C9ORF72

Antibodies are a key resource in biomedical research yet there are no community-accepted standards to rigorously characterize their quality. Here we develop a procedure to validate pre-existing antibodies. Human cell lines with high expression of a target, determined through a proteomics database, a...

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Bibliographic Details
Main Authors: Carl Laflamme, Paul M McKeever, Rahul Kumar, Julie Schwartz, Mahshad Kolahdouzan, Carol X Chen, Zhipeng You, Faiza Benaliouad, Opher Gileadi, Heidi M McBride, Thomas M Durcan, Aled M Edwards, Luke M Healy, Janice Robertson, Peter S McPherson
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2019-10-01
Series:eLife
Subjects:
amyotrophic lateral sclerosis
antibody
C9ORF72
macrophages
phagocytosis
lysosome
Online Access:https://elifesciences.org/articles/48363

Internet

https://elifesciences.org/articles/48363

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