FlsnRNA-seq: protoplasting-free full-length single-nucleus RNA profiling in plants

FlsnRNA-seq: protoplasting-free full-length single-nucleus RNA profiling in plants

Abstract The broad application of single-cell RNA profiling in plants has been hindered by the prerequisite of protoplasting that requires digesting the cell walls from different types of plant tissues. Here, we present a protoplasting-free approach, flsnRNA-seq, for large-scale full-length RNA prof...

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Bibliographic Details
Main Authors: Yanping Long, Zhijian Liu, Jinbu Jia, Weipeng Mo, Liang Fang, Dongdong Lu, Bo Liu, Hong Zhang, Wei Chen, Jixian Zhai
Format: Article
Language:English
Published: BMC 2021-02-01
Series:Genome Biology
Subjects:
Nanopore sequencing
Single-nucleus RNA-seq
Long-read
Online Access:https://doi.org/10.1186/s13059-021-02288-0

Internet

https://doi.org/10.1186/s13059-021-02288-0

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