Refinement of the Diatom Episome Maintenance Sequence and Improvement of Conjugation-based DNA Delivery Methods

Conjugation of episomal plasmids from bacteria to diatoms advances diatom genetic manipulation by simplifying transgene delivery and providing a stable and consistent gene expression platform. To reach its full potential, this nascent technology requires new optimized expression vectors and a deeper...

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Bibliographic Details
Main Authors: Rachel E Diner, Vincent A Bielinski, Chris Dupont, Andrew E Allen, Philip D Weyman
Format: Article
Language:English
Published: Frontiers Media S.A. 2016-08-01
Series:Frontiers in Bioengineering and Biotechnology
Subjects:
Online Access:http://journal.frontiersin.org/Journal/10.3389/fbioe.2016.00065/full