Developing transgenic wheat to encounter rusts and powdery mildew by overexpressing barley chi26 gene for fungal resistance

Developing transgenic wheat to encounter rusts and powdery mildew by overexpressing barley chi26 gene for fungal resistance

Abstract Background The main aim of this study was to improve fungal resistance in bread wheat via transgenesis. Transgenic wheat plants harboring barley chitinase (chi26) gene, driven by maize ubi promoter, were obtained using biolistic bombardment, whereas the herbicide resistance gene, bar, drive...

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Bibliographic Details
Main Authors: Hala F. Eissa, Sameh E. Hassanien, Ahmed M. Ramadan, Moustafa M. El-Shamy, Osama M. Saleh, Ahmed M. Shokry, Mohamed Abdelsattar, Yasser B. Morsy, Maher A. El-Maghraby, Hussien F. Alameldin, Sabah M. Hassan, Gamal H. Osman, Hesham T. Mahfouz, Gharib A. Gad El-Karim, Magdy A. Madkour, Ahmed Bahieldin
Format: Article
Language:English
Published: BMC 2017-05-01
Series:Plant Methods
Subjects:
Transgenesis
Southern
qPCR
Chitinase activity
qRT-PCR
Substantial equivalence
Online Access:http://link.springer.com/article/10.1186/s13007-017-0191-5

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http://link.springer.com/article/10.1186/s13007-017-0191-5

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