Targeted mutagenesis using the Agrobacterium tumefaciens-mediated CRISPR-Cas9 system in common wheat

Targeted mutagenesis using the Agrobacterium tumefaciens-mediated CRISPR-Cas9 system in common wheat

Abstract Background Recently, the CRISPR/Cas9 system has been widely used to precisely edit plant genomes. Due to the difficulty in Agrobacterium-mediated genetic transformation of wheat, the reported applications in CRISPR/Cas9 system were all based on the biolistic transformation. Results In the p...

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Bibliographic Details
Main Authors: Shujuan Zhang, Rongzhi Zhang, Guoqi Song, Jie Gao, Wei Li, Xiaodong Han, Mingli Chen, Yulian Li, Genying Li
Format: Article
Language:English
Published: BMC 2018-11-01
Series:BMC Plant Biology
Subjects:
Agrobacterium tumefaciens transformation
CRISPR/Cas9
Gene editing
Protoplast
Wheat
Online Access:http://link.springer.com/article/10.1186/s12870-018-1496-x

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http://link.springer.com/article/10.1186/s12870-018-1496-x

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