Multiplexed reverse transcription real-time polymerase chain reaction for simultaneous detection of Mayaro, Oropouche, and Oropouche-like viruses

Multiplexed reverse transcription real-time polymerase chain reaction for simultaneous detection of Mayaro, Oropouche, and Oropouche-like viruses

ABSTRACT We describe a sensitive method for simultaneous detection of Oropouche and Oropouche-like viruses carrying the Oropouche S segment, as well as the Mayaro virus, using a multiplexed one-step reverse transcription real-time polymerase chain reaction (RT-qPCR). A chimeric plasmid containing bo...

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Bibliographic Details
Main Authors: Felipe Gomes Naveca, Valdinete Alves do Nascimento, Victor Costa de Souza, Bruno Tardelli Diniz Nunes, Daniela Sueli Guerreiro Rodrigues, Pedro Fernando da Costa Vasconcelos
Format: Article
Language:English
Published: Instituto Oswaldo Cruz, Ministério da Saúde
Series:Memórias do Instituto Oswaldo Cruz.
Subjects:
real-time PCR
Mayaro virus
Oropouche virus
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Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762017000700510&lng=en&tlng=en

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http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762017000700510&lng=en&tlng=en

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