Monitoring cell-mediated immune responses in AAV gene therapy clinical trials using a validated IFN-γ ELISpot method

Monitoring cell-mediated immune responses in AAV gene therapy clinical trials using a validated IFN-γ ELISpot method

Adeno-associated virus (AAV)-based gene therapies have recently shown promise as a novel treatment for hereditary diseases. Due to the viral origin of the vector capsid, however, cellular immune response may be elicited that could eliminate transduced target cells. To monitor cellular immune respons...

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Bibliographic Details
Main Authors: Kathryn S. Patton, M. Travis Harrison, Brian R. Long, Kelly Lau, Jennifer Holcomb, Rachel Owen, Theresa Kasprzyk, Sylvia Janetzki, Stephen J. Zoog, Christian Vettermann
Format: Article
Language:English
Published: Elsevier 2021-09-01
Series:Molecular Therapy: Methods & Clinical Development
Subjects:
IFN-γ cytokine secretion
ELISpot assay
T cell immunity
method performance validation
AAV5-FVIII gene therapy
hemophilia
Online Access:http://www.sciencedirect.com/science/article/pii/S2329050121000966

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http://www.sciencedirect.com/science/article/pii/S2329050121000966

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