Characterization of antigen-specific B cells using nominal antigen-coated flow-beads.

Characterization of antigen-specific B cells using nominal antigen-coated flow-beads.

In order to characterize the reactivity of B cells against nominal antigens, a method based on the coupling of antigens onto the surface of fluorescent core polystyrene beads was developed. We first demonstrate that murine B cells with a human MOG-specific BCR are able to interact with MOG-coated be...

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Bibliographic Details
Main Authors: Nicolas Degauque, Annie Elong Ngono, Ahmed Akl, Maud Lepetit, Romain Crochette, Magali Giral, Julie Lepourry, Annaick Pallier, Stéphanie Castagnet, Emilie Dugast, Cécile Guillot-Gueguen, Marylène Jacq-Foucher, Xavier Saulquin, Anne Cesbron, David Laplaud, Arnaud Nicot, Sophie Brouard, Jean-Paul Soulillou
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3875494?pdf=render

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http://europepmc.org/articles/PMC3875494?pdf=render

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