A Bit Stickier, a Bit Slower, a Lot Stiffer: Specific vs. Nonspecific Binding of Gal4 to DNA

A Bit Stickier, a Bit Slower, a Lot Stiffer: Specific vs. Nonspecific Binding of Gal4 to DNA

Transcription factors regulate gene activity by binding specific regions of genomic DNA thanks to a subtle interplay of specific and nonspecific interactions that is challenging to quantify. Here, we exploit Reflective Phantom Interface (RPI), a label-free biosensor based on optical reflectivity, to...

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Bibliographic Details
Main Authors: Thomas Carzaniga, Giuliano Zanchetta, Elisa Frezza, Luca Casiraghi, Luka Vanjur, Giovanni Nava, Giovanni Tagliabue, Giorgio Dieci, Marco Buscaglia, Tommaso Bellini
Format: Article
Language:English
Published: MDPI AG 2021-04-01
Series:International Journal of Molecular Sciences
Subjects:
transcription factors
biosensor
protein–DNA interactions
molecular dynamics
biophysics
entropy–enthalpy compensation
Online Access:https://www.mdpi.com/1422-0067/22/8/3813

Internet

https://www.mdpi.com/1422-0067/22/8/3813

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