Erythropoietin production by the kidney and the liver in response to severe hypoxia evaluated by Western blotting with deglycosylation

Erythropoietin production by the kidney and the liver in response to severe hypoxia evaluated by Western blotting with deglycosylation

Abstract The detection of erythropoietin (Epo) protein by Western blotting has required pre‐purification of the sample. We developed a new Western blot method to detect plasma and urinary Epo using deglycosylation. Epo in urine and tissue, and erythropoiesis‐stimulating agents (ESAs) in urine were d...

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Bibliographic Details
Main Authors: Yukiko Yasuoka, Takashi Fukuyama, Yuichiro Izumi, Yushi Nakayama, Hideki Inoue, Kengo Yanagita, Tomomi Oshima, Taiga Yamazaki, Takayuki Uematsu, Noritada Kobayashi, Yoshitaka Shimada, Yasushi Nagaba, Masashi Mukoyama, Tetsuro Yamashita, Yuichi Sato, Jeff M. Sands, Katsumasa Kawahara, Hiroshi Nonoguchi
Format: Article
Language:English
Published: Wiley 2020-06-01
Series:Physiological Reports
Subjects:
anemia
deglycosylation
erythropoiesis‐stimulating agents
erythropoietin
hypoxia
Online Access:https://doi.org/10.14814/phy2.14485

Internet

https://doi.org/10.14814/phy2.14485

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