A protocol for the systematic review and meta-analysis of thigmotactic behaviour in the open field test in rodent models associated with persistent pain
Objective Thigmotaxis is an innate predator avoidance behaviour of rodents and is enhanced when animals are under stress. It is characterised by the preference of a rodent to seek shelter, rather than expose itself to the aversive open area. The behaviour has been proposed to be a measurable constru...
Main Authors: | , , |
---|---|
Format: | Article |
Language: | English |
Published: |
BMJ Publishing Group
2021-07-01
|
Series: | BMJ Open Science |
Online Access: | https://openscience.bmj.com/content/5/1/e100135.full |
id |
doaj-5edd325ae8cb474297f979fda2bbc054 |
---|---|
record_format |
Article |
spelling |
doaj-5edd325ae8cb474297f979fda2bbc0542021-07-28T16:00:07ZengBMJ Publishing GroupBMJ Open Science2398-87032021-07-015110.1136/bmjos-2020-100135A protocol for the systematic review and meta-analysis of thigmotactic behaviour in the open field test in rodent models associated with persistent painJan Vollert0Xue Ying Zhang1Andrew SC Rice2Musculoskeletal, Imperial College London, London, UKMusculoskeletal, Imperial College London, London, UKMusculoskeletal, Imperial College London, London, UKObjective Thigmotaxis is an innate predator avoidance behaviour of rodents and is enhanced when animals are under stress. It is characterised by the preference of a rodent to seek shelter, rather than expose itself to the aversive open area. The behaviour has been proposed to be a measurable construct that can address the impact of pain on rodent behaviour. This systematic review will assess whether thigmotaxis can be influenced by experimental persistent pain and attenuated by pharmacological interventions in rodents.Search strategy We will conduct search on three electronic databases to identify studies in which thigmotaxis was used as an outcome measure contextualised to a rodent model associated with persistent pain. All studies published until the date of the search will be considered.Screening and annotation Two independent reviewers will screen studies based on the order of (1) titles and abstracts, and (2) full texts.Data management and reporting For meta-analysis, we will extract thigmotactic behavioural data and calculate effect sizes. Effect sizes will be combined using a random-effects model. We will assess heterogeneity and identify sources of heterogeneity. A risk-of-bias assessment will be conducted to evaluate study quality. Publication bias will be assessed using funnel plots, Egger’s regression and trim-and-fill analysis. We will also extract stimulus-evoked limb withdrawal data to assess its correlation with thigmotaxis in the same animals. The evidence obtained will provide a comprehensive understanding of the strengths and limitations of using thigmotactic outcome measure in animal pain research so that future experimental designs can be optimised. We will follow the Preferred Reporting Items for Systematic Reviews and Meta-Analyses reporting guidelines and disseminate the review findings through publication and conference presentation.https://openscience.bmj.com/content/5/1/e100135.full |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jan Vollert Xue Ying Zhang Andrew SC Rice |
spellingShingle |
Jan Vollert Xue Ying Zhang Andrew SC Rice A protocol for the systematic review and meta-analysis of thigmotactic behaviour in the open field test in rodent models associated with persistent pain BMJ Open Science |
author_facet |
Jan Vollert Xue Ying Zhang Andrew SC Rice |
author_sort |
Jan Vollert |
title |
A protocol for the systematic review and meta-analysis of thigmotactic behaviour in the open field test in rodent models associated with persistent pain |
title_short |
A protocol for the systematic review and meta-analysis of thigmotactic behaviour in the open field test in rodent models associated with persistent pain |
title_full |
A protocol for the systematic review and meta-analysis of thigmotactic behaviour in the open field test in rodent models associated with persistent pain |
title_fullStr |
A protocol for the systematic review and meta-analysis of thigmotactic behaviour in the open field test in rodent models associated with persistent pain |
title_full_unstemmed |
A protocol for the systematic review and meta-analysis of thigmotactic behaviour in the open field test in rodent models associated with persistent pain |
title_sort |
protocol for the systematic review and meta-analysis of thigmotactic behaviour in the open field test in rodent models associated with persistent pain |
publisher |
BMJ Publishing Group |
series |
BMJ Open Science |
issn |
2398-8703 |
publishDate |
2021-07-01 |
description |
Objective Thigmotaxis is an innate predator avoidance behaviour of rodents and is enhanced when animals are under stress. It is characterised by the preference of a rodent to seek shelter, rather than expose itself to the aversive open area. The behaviour has been proposed to be a measurable construct that can address the impact of pain on rodent behaviour. This systematic review will assess whether thigmotaxis can be influenced by experimental persistent pain and attenuated by pharmacological interventions in rodents.Search strategy We will conduct search on three electronic databases to identify studies in which thigmotaxis was used as an outcome measure contextualised to a rodent model associated with persistent pain. All studies published until the date of the search will be considered.Screening and annotation Two independent reviewers will screen studies based on the order of (1) titles and abstracts, and (2) full texts.Data management and reporting For meta-analysis, we will extract thigmotactic behavioural data and calculate effect sizes. Effect sizes will be combined using a random-effects model. We will assess heterogeneity and identify sources of heterogeneity. A risk-of-bias assessment will be conducted to evaluate study quality. Publication bias will be assessed using funnel plots, Egger’s regression and trim-and-fill analysis. We will also extract stimulus-evoked limb withdrawal data to assess its correlation with thigmotaxis in the same animals. The evidence obtained will provide a comprehensive understanding of the strengths and limitations of using thigmotactic outcome measure in animal pain research so that future experimental designs can be optimised. We will follow the Preferred Reporting Items for Systematic Reviews and Meta-Analyses reporting guidelines and disseminate the review findings through publication and conference presentation. |
url |
https://openscience.bmj.com/content/5/1/e100135.full |
work_keys_str_mv |
AT janvollert aprotocolforthesystematicreviewandmetaanalysisofthigmotacticbehaviourintheopenfieldtestinrodentmodelsassociatedwithpersistentpain AT xueyingzhang aprotocolforthesystematicreviewandmetaanalysisofthigmotacticbehaviourintheopenfieldtestinrodentmodelsassociatedwithpersistentpain AT andrewscrice aprotocolforthesystematicreviewandmetaanalysisofthigmotacticbehaviourintheopenfieldtestinrodentmodelsassociatedwithpersistentpain AT janvollert protocolforthesystematicreviewandmetaanalysisofthigmotacticbehaviourintheopenfieldtestinrodentmodelsassociatedwithpersistentpain AT xueyingzhang protocolforthesystematicreviewandmetaanalysisofthigmotacticbehaviourintheopenfieldtestinrodentmodelsassociatedwithpersistentpain AT andrewscrice protocolforthesystematicreviewandmetaanalysisofthigmotacticbehaviourintheopenfieldtestinrodentmodelsassociatedwithpersistentpain |
_version_ |
1721268066379104256 |