Correlation of Real Time PCR Cycle Threshold Cut-Off with Bordetella pertussis Clinical Severity.

Bordetella pertussis testing performed using real-time polymerase chain reaction (RT-PCR) is interpreted based on a cycle threshold (Ct) value. At Public Health Ontario Laboratories (PHOL), a Ct value <36 is reported as positive, and Ct values ≥36 and <40 are reported as indeterminate. PHOL re...

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Bibliographic Details
Main Authors: Shelly Bolotin, Shelley L Deeks, Alex Marchand-Austin, Heather Rilkoff, Vica Dang, Ryan Walton, Ahmed Hashim, David Farrell, Natasha S Crowcroft
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2015-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0133209