Tonically Active α2 Subunit-Containing Glycine Receptors Regulate the Excitability of Striatal Medium Spiny Neurons
Medium spiny neurons (MSNs) of the dorsal striatum represent the first relay of cortico–striato–thalamic loop, responsible for the initiation of voluntary movements and motor learning. GABAergic transmission exerts the main inhibitory control of MSNs. However, MSNs also express chloride-permeable gl...
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doaj-5ee5c682663e461086de2ebe2b7ec1532020-11-24T23:08:22ZengFrontiers Media S.A.Frontiers in Molecular Neuroscience1662-50992018-01-011010.3389/fnmol.2017.00442316936Tonically Active α2 Subunit-Containing Glycine Receptors Regulate the Excitability of Striatal Medium Spiny NeuronsSvetlana M. Molchanova0Joris Comhair1Joris Comhair2Deniz Karadurmus3Elisabeth Piccart4Robert J. Harvey5Robert J. Harvey6Jean-Michel Rigo7Serge N. Schiffmann8Bert Brône9David Gall10Laboratory of Neurophysiology, ULB Neuroscience Institute, Université Libre de Bruxelles (ULB), Brussels, BelgiumLaboratory of Neurophysiology, ULB Neuroscience Institute, Université Libre de Bruxelles (ULB), Brussels, BelgiumBiomedical Research Institute, University of Hasselt (UHasselt), Hasselt, BelgiumLaboratory of Neurophysiology, ULB Neuroscience Institute, Université Libre de Bruxelles (ULB), Brussels, BelgiumBiomedical Research Institute, University of Hasselt (UHasselt), Hasselt, BelgiumSchool of Health and Sport Sciences, University of the Sunshine Coast, Sippy Downs, QLD, AustraliaSunshine Coast Health Institute, Birtinya, QLD, AustraliaBiomedical Research Institute, University of Hasselt (UHasselt), Hasselt, BelgiumLaboratory of Neurophysiology, ULB Neuroscience Institute, Université Libre de Bruxelles (ULB), Brussels, BelgiumBiomedical Research Institute, University of Hasselt (UHasselt), Hasselt, BelgiumLaboratory of Neurophysiology, ULB Neuroscience Institute, Université Libre de Bruxelles (ULB), Brussels, BelgiumMedium spiny neurons (MSNs) of the dorsal striatum represent the first relay of cortico–striato–thalamic loop, responsible for the initiation of voluntary movements and motor learning. GABAergic transmission exerts the main inhibitory control of MSNs. However, MSNs also express chloride-permeable glycine receptors (GlyRs) although their subunit composition and functional significance in the striatum is unknown. Here, we studied the function of GlyRs in MSNs of young adult mice. We show that MSNs express functional GlyRs, with α2 being the main agonist binding subunit. These receptors are extrasynaptic and depolarizing at resting state. The pharmacological inhibition of GlyRs, as well as inactivation of the GlyR α2 subunit gene hyperpolarize the membrane potential of MSNs and increase their action potential firing offset. Mice lacking GlyR α2 showed impaired motor memory consolidation without any changes in the initial motor performance. Taken together, these results demonstrate that tonically active GlyRs regulate the firing properties of MSNs and may thus affect the function of basal ganglia.http://journal.frontiersin.org/article/10.3389/fnmol.2017.00442/fulldorsal striatummedium spiny neuronsglycine receptorstonic currentexcitability |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Svetlana M. Molchanova Joris Comhair Joris Comhair Deniz Karadurmus Elisabeth Piccart Robert J. Harvey Robert J. Harvey Jean-Michel Rigo Serge N. Schiffmann Bert Brône David Gall |
spellingShingle |
Svetlana M. Molchanova Joris Comhair Joris Comhair Deniz Karadurmus Elisabeth Piccart Robert J. Harvey Robert J. Harvey Jean-Michel Rigo Serge N. Schiffmann Bert Brône David Gall Tonically Active α2 Subunit-Containing Glycine Receptors Regulate the Excitability of Striatal Medium Spiny Neurons Frontiers in Molecular Neuroscience dorsal striatum medium spiny neurons glycine receptors tonic current excitability |
author_facet |
Svetlana M. Molchanova Joris Comhair Joris Comhair Deniz Karadurmus Elisabeth Piccart Robert J. Harvey Robert J. Harvey Jean-Michel Rigo Serge N. Schiffmann Bert Brône David Gall |
author_sort |
Svetlana M. Molchanova |
title |
Tonically Active α2 Subunit-Containing Glycine Receptors Regulate the Excitability of Striatal Medium Spiny Neurons |
title_short |
Tonically Active α2 Subunit-Containing Glycine Receptors Regulate the Excitability of Striatal Medium Spiny Neurons |
title_full |
Tonically Active α2 Subunit-Containing Glycine Receptors Regulate the Excitability of Striatal Medium Spiny Neurons |
title_fullStr |
Tonically Active α2 Subunit-Containing Glycine Receptors Regulate the Excitability of Striatal Medium Spiny Neurons |
title_full_unstemmed |
Tonically Active α2 Subunit-Containing Glycine Receptors Regulate the Excitability of Striatal Medium Spiny Neurons |
title_sort |
tonically active α2 subunit-containing glycine receptors regulate the excitability of striatal medium spiny neurons |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Molecular Neuroscience |
issn |
1662-5099 |
publishDate |
2018-01-01 |
description |
Medium spiny neurons (MSNs) of the dorsal striatum represent the first relay of cortico–striato–thalamic loop, responsible for the initiation of voluntary movements and motor learning. GABAergic transmission exerts the main inhibitory control of MSNs. However, MSNs also express chloride-permeable glycine receptors (GlyRs) although their subunit composition and functional significance in the striatum is unknown. Here, we studied the function of GlyRs in MSNs of young adult mice. We show that MSNs express functional GlyRs, with α2 being the main agonist binding subunit. These receptors are extrasynaptic and depolarizing at resting state. The pharmacological inhibition of GlyRs, as well as inactivation of the GlyR α2 subunit gene hyperpolarize the membrane potential of MSNs and increase their action potential firing offset. Mice lacking GlyR α2 showed impaired motor memory consolidation without any changes in the initial motor performance. Taken together, these results demonstrate that tonically active GlyRs regulate the firing properties of MSNs and may thus affect the function of basal ganglia. |
topic |
dorsal striatum medium spiny neurons glycine receptors tonic current excitability |
url |
http://journal.frontiersin.org/article/10.3389/fnmol.2017.00442/full |
work_keys_str_mv |
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