A two-hybrid assay to study protein interactions within the secretory pathway.

A two-hybrid assay to study protein interactions within the secretory pathway.

Interactions of transcriptional activators are difficult to study using transcription-based two-hybrid assays due to potent activation resulting in false positives. Here we report the development of the Golgi two-hybrid (G2H), a method that interrogates protein interactions within the Golgi, where t...

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Bibliographic Details
Main Authors: Danielle H Dube, Bin Li, Ethan J Greenblatt, Sadeieh Nimer, Amanda K Raymond, Jennifer J Kohler
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2010-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3011011?pdf=render

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http://europepmc.org/articles/PMC3011011?pdf=render

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