Endotoxin-free purification for the isolation of Bovine Viral Diarrhoea Virus E2 protein from insoluble inclusion body aggregates

<p>Abstract</p> <p>Background</p> <p>Protein expression in <it>Escherichia coli </it>may result in the recombinant protein being expressed as insoluble inclusion bodies. In addition, proteins purified from <it>E. coli </it>contain endotoxins whic...

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Bibliographic Details
Main Authors: Mahony Timothy J, Commins Margaret, Mahony Donna, Cavallaro Antonino S, Mitter Neena
Format: Article
Language:English
Published: BMC 2011-07-01
Series:Microbial Cell Factories
Online Access:http://www.microbialcellfactories.com/content/10/1/57