In trans paired nicking triggers seamless genome editing without double-stranded DNA cutting

In trans paired nicking triggers seamless genome editing without double-stranded DNA cutting

CRISPR-Cas9-based gene editing involves double-strand breaks at target sequences, which are often repaired by mutagenic non-homologous end-joining. Here the authors use Cas9 nickases to generate coordinated single-strand breaks in donor and target DNA for precise homology-directed gene editing.

Bibliographic Details
Main Authors: Xiaoyu Chen, Josephine M. Janssen, Jin Liu, Ignazio Maggio, Anke E. J. ‘t Jong, Harald M.M. Mikkers, Manuel A. F. V. Gonçalves
Format: Article
Language:English
Published: Nature Publishing Group 2017-09-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-017-00687-1

Internet

https://doi.org/10.1038/s41467-017-00687-1

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