In trans paired nicking triggers seamless genome editing without double-stranded DNA cutting
CRISPR-Cas9-based gene editing involves double-strand breaks at target sequences, which are often repaired by mutagenic non-homologous end-joining. Here the authors use Cas9 nickases to generate coordinated single-strand breaks in donor and target DNA for precise homology-directed gene editing.
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Publishing Group
2017-09-01
|
| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-017-00687-1 |
| id |
doaj-60102009427545b88a37146eedf8137c |
|---|---|
| record_format |
Article |
| spelling |
doaj-60102009427545b88a37146eedf8137c2021-05-11T07:47:10ZengNature Publishing GroupNature Communications2041-17232017-09-018111510.1038/s41467-017-00687-1In trans paired nicking triggers seamless genome editing without double-stranded DNA cuttingXiaoyu Chen0Josephine M. Janssen1Jin Liu2Ignazio Maggio3Anke E. J. ‘t Jong4Harald M.M. Mikkers5Manuel A. F. V. Gonçalves6Department of Molecular Cell Biology, Leiden University Medical CenterDepartment of Molecular Cell Biology, Leiden University Medical CenterDepartment of Molecular Cell Biology, Leiden University Medical CenterDepartment of Molecular Cell Biology, Leiden University Medical CenterDepartment of Molecular Cell Biology, Leiden University Medical CenterDepartment of Molecular Cell Biology, Leiden University Medical CenterDepartment of Molecular Cell Biology, Leiden University Medical CenterCRISPR-Cas9-based gene editing involves double-strand breaks at target sequences, which are often repaired by mutagenic non-homologous end-joining. Here the authors use Cas9 nickases to generate coordinated single-strand breaks in donor and target DNA for precise homology-directed gene editing.https://doi.org/10.1038/s41467-017-00687-1 |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Xiaoyu Chen Josephine M. Janssen Jin Liu Ignazio Maggio Anke E. J. ‘t Jong Harald M.M. Mikkers Manuel A. F. V. Gonçalves |
| spellingShingle |
Xiaoyu Chen Josephine M. Janssen Jin Liu Ignazio Maggio Anke E. J. ‘t Jong Harald M.M. Mikkers Manuel A. F. V. Gonçalves In trans paired nicking triggers seamless genome editing without double-stranded DNA cutting Nature Communications |
| author_facet |
Xiaoyu Chen Josephine M. Janssen Jin Liu Ignazio Maggio Anke E. J. ‘t Jong Harald M.M. Mikkers Manuel A. F. V. Gonçalves |
| author_sort |
Xiaoyu Chen |
| title |
In trans paired nicking triggers seamless genome editing without double-stranded DNA cutting |
| title_short |
In trans paired nicking triggers seamless genome editing without double-stranded DNA cutting |
| title_full |
In trans paired nicking triggers seamless genome editing without double-stranded DNA cutting |
| title_fullStr |
In trans paired nicking triggers seamless genome editing without double-stranded DNA cutting |
| title_full_unstemmed |
In trans paired nicking triggers seamless genome editing without double-stranded DNA cutting |
| title_sort |
in trans paired nicking triggers seamless genome editing without double-stranded dna cutting |
| publisher |
Nature Publishing Group |
| series |
Nature Communications |
| issn |
2041-1723 |
| publishDate |
2017-09-01 |
| description |
CRISPR-Cas9-based gene editing involves double-strand breaks at target sequences, which are often repaired by mutagenic non-homologous end-joining. Here the authors use Cas9 nickases to generate coordinated single-strand breaks in donor and target DNA for precise homology-directed gene editing. |
| url |
https://doi.org/10.1038/s41467-017-00687-1 |
| work_keys_str_mv |
AT xiaoyuchen intranspairednickingtriggersseamlessgenomeeditingwithoutdoublestrandeddnacutting AT josephinemjanssen intranspairednickingtriggersseamlessgenomeeditingwithoutdoublestrandeddnacutting AT jinliu intranspairednickingtriggersseamlessgenomeeditingwithoutdoublestrandeddnacutting AT ignaziomaggio intranspairednickingtriggersseamlessgenomeeditingwithoutdoublestrandeddnacutting AT ankeejtjong intranspairednickingtriggersseamlessgenomeeditingwithoutdoublestrandeddnacutting AT haraldmmmikkers intranspairednickingtriggersseamlessgenomeeditingwithoutdoublestrandeddnacutting AT manuelafvgoncalves intranspairednickingtriggersseamlessgenomeeditingwithoutdoublestrandeddnacutting |
| _version_ |
1721451633516216320 |