A highly sensitive method for analysis of 7-dehydrocholesterol for the study of Smith-Lemli-Opitz syndrome[S]

A highly sensitive method for analysis of 7-dehydrocholesterol for the study of Smith-Lemli-Opitz syndrome[S]

We describe a highly sensitive method for the detection of 7-dehydrocholesterol (7-DHC), the biosynthetic precursor of cholesterol, based on its reactivity with 4-phenyl-1,2,4-triazoline-3,5-dione (PTAD) in a Diels-Alder cycloaddition reaction. Samples of biological tissues and fluids with added deu...

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Bibliographic Details
Main Authors: Wei Liu, Libin Xu, Connor Lamberson, Dorothea Haas, Zeljka Korade, Ned A. Porter
Format: Article
Language:English
Published: Elsevier 2014-02-01
Series:Journal of Lipid Research
Subjects:
desmosterol
Diels-Alder cycloaddition
PTAD
liquid chromatography-mass spectrometry
gas chromatography
7-dehydrocholesterol reductase
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520350410

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http://www.sciencedirect.com/science/article/pii/S0022227520350410

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