Statistical optimization for tannase production by Aspergillus tubingensis in solid-state fermentation using tea stalks
Background: A sequential statistical strategy was used to optimize tannase production from Aspergillus tubingensis using tea stalks by solid-state fermentation. Results: First, using a Plackett–Burman design, inoculum size and incubation time (among seven tested variables) were identified as the mos...
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Elsevier
2015-05-01
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| Series: | Electronic Journal of Biotechnology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S071734581500024X |
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doaj-60327216891c4f30a265afa11c0d2b502020-11-24T23:17:49ZengElsevierElectronic Journal of Biotechnology0717-34582015-05-0118314314710.1016/j.ejbt.2015.02.001Statistical optimization for tannase production by Aspergillus tubingensis in solid-state fermentation using tea stalksAnfeng Xiao0Yufeng Huang1Hui Ni2Huinong Cai3Qiuming Yang4College of Food and Biological Engineering, Jimei University, Xiamen 361021, ChinaCollege of Food and Biological Engineering, Jimei University, Xiamen 361021, ChinaCollege of Food and Biological Engineering, Jimei University, Xiamen 361021, ChinaCollege of Food and Biological Engineering, Jimei University, Xiamen 361021, ChinaCollege of Food and Biological Engineering, Jimei University, Xiamen 361021, ChinaBackground: A sequential statistical strategy was used to optimize tannase production from Aspergillus tubingensis using tea stalks by solid-state fermentation. Results: First, using a Plackett–Burman design, inoculum size and incubation time (among seven tested variables) were identified as the most significant factors for tannase yield. The effects of significant variables were further evaluated through a single steepest ascent experiment and central composite design with response surface analysis. Under optimal conditions, the experimental value of 84.24 units per gram of dry substrate (U/gds) closely matched the predicted value of 87.26 U/gds. Conclusions: The result of the statistical approach was 2.09 times higher than the basal medium (40.22 U/gds). The results were fitted onto a second-order polynomial model with a correlation coefficient (R2) of 0.9340, which implied an adequate credibility of the model.http://www.sciencedirect.com/science/article/pii/S071734581500024XAspergillus tubingensisProcess optimizationResponse surface methodologyTea stalksTannase fermentation |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Anfeng Xiao Yufeng Huang Hui Ni Huinong Cai Qiuming Yang |
| spellingShingle |
Anfeng Xiao Yufeng Huang Hui Ni Huinong Cai Qiuming Yang Statistical optimization for tannase production by Aspergillus tubingensis in solid-state fermentation using tea stalks Electronic Journal of Biotechnology Aspergillus tubingensis Process optimization Response surface methodology Tea stalks Tannase fermentation |
| author_facet |
Anfeng Xiao Yufeng Huang Hui Ni Huinong Cai Qiuming Yang |
| author_sort |
Anfeng Xiao |
| title |
Statistical optimization for tannase production by Aspergillus tubingensis in solid-state fermentation using tea stalks |
| title_short |
Statistical optimization for tannase production by Aspergillus tubingensis in solid-state fermentation using tea stalks |
| title_full |
Statistical optimization for tannase production by Aspergillus tubingensis in solid-state fermentation using tea stalks |
| title_fullStr |
Statistical optimization for tannase production by Aspergillus tubingensis in solid-state fermentation using tea stalks |
| title_full_unstemmed |
Statistical optimization for tannase production by Aspergillus tubingensis in solid-state fermentation using tea stalks |
| title_sort |
statistical optimization for tannase production by aspergillus tubingensis in solid-state fermentation using tea stalks |
| publisher |
Elsevier |
| series |
Electronic Journal of Biotechnology |
| issn |
0717-3458 |
| publishDate |
2015-05-01 |
| description |
Background: A sequential statistical strategy was used to optimize tannase production from Aspergillus tubingensis using tea stalks by solid-state fermentation.
Results: First, using a Plackett–Burman design, inoculum size and incubation time (among seven tested variables) were identified as the most significant factors for tannase yield. The effects of significant variables were further evaluated through a single steepest ascent experiment and central composite design with response surface analysis. Under optimal conditions, the experimental value of 84.24 units per gram of dry substrate (U/gds) closely matched the predicted value of 87.26 U/gds.
Conclusions: The result of the statistical approach was 2.09 times higher than the basal medium (40.22 U/gds). The results were fitted onto a second-order polynomial model with a correlation coefficient (R2) of 0.9340, which implied an adequate credibility of the model. |
| topic |
Aspergillus tubingensis Process optimization Response surface methodology Tea stalks Tannase fermentation |
| url |
http://www.sciencedirect.com/science/article/pii/S071734581500024X |
| work_keys_str_mv |
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