An expanded palette of improved SPLICS reporters detects multiple organelle contacts in vitro and in vivo

The authors have previously reported split-GFP-based contact site sensors (SPLICS) to document endoplasmic reticulum/mitochondria contact sites. Here they extend this work and develop a range of improved SPLICS sensors to detect single and multiple organelle contact sites at different distances.

Bibliographic Details
Main Authors:	Francesca Vallese, Cristina Catoni, Domenico Cieri, Lucia Barazzuol, Omar Ramirez, Valentina Calore, Massimo Bonora, Flavia Giamogante, Paolo Pinton, Marisa Brini, Tito Calì
Format:	Article
Language:	English
Published:	Nature Publishing Group 2020-11-01
Series:	Nature Communications
Online Access:	https://doi.org/10.1038/s41467-020-19892-6

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spelling	doaj-605e86eacf834bb7bdf8a03fe68ceb5e2021-05-11T09:16:35ZengNature Publishing GroupNature Communications2041-17232020-11-0111111510.1038/s41467-020-19892-6An expanded palette of improved SPLICS reporters detects multiple organelle contacts in vitro and in vivoFrancesca Vallese0Cristina Catoni1Domenico Cieri2Lucia Barazzuol3Omar Ramirez4Valentina Calore5Massimo Bonora6Flavia Giamogante7Paolo Pinton8Marisa Brini9Tito Calì10Department of Biomedical Sciences, University of PadovaDepartment of Biology, University of PadovaDepartment of Biomedical Sciences, University of PadovaDepartment of Biomedical Sciences, University of PadovaDepartment of Neurobiology, Interdisciplinary Center for Neurosciences, Heidelberg UniversityDepartment of Biomedical Sciences, University of PadovaDepartment of Morphology, Surgery and Experimental Medicine, Section of General Pathology, University of FerraraDepartment of Biomedical Sciences, University of PadovaDepartment of Morphology, Surgery and Experimental Medicine, Section of General Pathology, University of FerraraDepartment of Biology, University of PadovaDepartment of Biomedical Sciences, University of PadovaThe authors have previously reported split-GFP-based contact site sensors (SPLICS) to document endoplasmic reticulum/mitochondria contact sites. Here they extend this work and develop a range of improved SPLICS sensors to detect single and multiple organelle contact sites at different distances.https://doi.org/10.1038/s41467-020-19892-6
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Francesca Vallese Cristina Catoni Domenico Cieri Lucia Barazzuol Omar Ramirez Valentina Calore Massimo Bonora Flavia Giamogante Paolo Pinton Marisa Brini Tito Calì
spellingShingle	Francesca Vallese Cristina Catoni Domenico Cieri Lucia Barazzuol Omar Ramirez Valentina Calore Massimo Bonora Flavia Giamogante Paolo Pinton Marisa Brini Tito Calì An expanded palette of improved SPLICS reporters detects multiple organelle contacts in vitro and in vivo Nature Communications
author_facet	Francesca Vallese Cristina Catoni Domenico Cieri Lucia Barazzuol Omar Ramirez Valentina Calore Massimo Bonora Flavia Giamogante Paolo Pinton Marisa Brini Tito Calì
author_sort	Francesca Vallese
title	An expanded palette of improved SPLICS reporters detects multiple organelle contacts in vitro and in vivo
title_short	An expanded palette of improved SPLICS reporters detects multiple organelle contacts in vitro and in vivo
title_full	An expanded palette of improved SPLICS reporters detects multiple organelle contacts in vitro and in vivo
title_fullStr	An expanded palette of improved SPLICS reporters detects multiple organelle contacts in vitro and in vivo
title_full_unstemmed	An expanded palette of improved SPLICS reporters detects multiple organelle contacts in vitro and in vivo
title_sort	expanded palette of improved splics reporters detects multiple organelle contacts in vitro and in vivo
publisher	Nature Publishing Group
series	Nature Communications
issn	2041-1723
publishDate	2020-11-01
description	The authors have previously reported split-GFP-based contact site sensors (SPLICS) to document endoplasmic reticulum/mitochondria contact sites. Here they extend this work and develop a range of improved SPLICS sensors to detect single and multiple organelle contact sites at different distances.
url	https://doi.org/10.1038/s41467-020-19892-6
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An expanded palette of improved SPLICS reporters detects multiple organelle contacts in vitro and in vivo

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