An expanded palette of improved SPLICS reporters detects multiple organelle contacts in vitro and in vivo
The authors have previously reported split-GFP-based contact site sensors (SPLICS) to document endoplasmic reticulum/mitochondria contact sites. Here they extend this work and develop a range of improved SPLICS sensors to detect single and multiple organelle contact sites at different distances.
Main Authors: | Francesca Vallese, Cristina Catoni, Domenico Cieri, Lucia Barazzuol, Omar Ramirez, Valentina Calore, Massimo Bonora, Flavia Giamogante, Paolo Pinton, Marisa Brini, Tito Calì |
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Format: | Article |
Language: | English |
Published: |
Nature Publishing Group
2020-11-01
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Series: | Nature Communications |
Online Access: | https://doi.org/10.1038/s41467-020-19892-6 |
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