Measuring enzyme binding using shaped ultrafast laser pulses

We use multiphoton quantum-control spectroscopy to discriminate between enzyme-bound and unbound NADH (reduced nicotinamide adenine dinucleotide) molecules in solution. Shaped ultrafast laser pulses are used to illuminate both forms of NADH, and the ratio of the fluorescence from the bound and unbou...

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Bibliographic Details
Main Authors: Weinacht T. C., Tseng C.-H., Pearson B. J.
Format: Article
Language:English
Published: EDP Sciences 2013-03-01
Series:EPJ Web of Conferences
Online Access:http://dx.doi.org/10.1051/epjconf/20134107013