Effect of Lipoxin A4 on Lipopolysaccharide-Induced Endothelial Hyperpermeability
Excessive oxidative stress, decreased antioxidant capacity, and enhanced cellular calcium levels are initial factors that cause endothelial cell (EC) hyperpermeability, which represents a crucial event in the pathogenesis of pre-eclampsia. Lipoxin A4 (LXA4) strongly attenuated lipopolysaccharide (LP...
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doaj-60ae597c24d34f24995d9d13ed4776de2020-11-25T01:13:32ZengHindawi LimitedThe Scientific World Journal1537-744X2011-01-01111056106710.1100/tsw.2011.98Effect of Lipoxin A4 on Lipopolysaccharide-Induced Endothelial HyperpermeabilityHuayan Pang0Pan Yi1Ping Wu2Zhuoya Liu3Zhongjie Liu4Jianming Gong5Hua Hao6Lei Cai7Duyun Ye8Yinping Huang9Department of Obstetrics and Gynecology, First Affiliated Hospital of Wenzhou Medical College, Wenzhou, Zhejiang, ChinaDepartment of Pathophysiology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, ChinaDepartment of Pathophysiology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, ChinaDepartment of Pathophysiology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, ChinaDepartment of Obstetrics and Gynecology, First Affiliated Hospital of Wenzhou Medical College, Wenzhou, Zhejiang, ChinaDepartment of Obstetrics and Gynecology, First Affiliated Hospital of Wenzhou Medical College, Wenzhou, Zhejiang, ChinaDepartment of Pathophysiology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, ChinaDepartment of Pathophysiology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, ChinaDepartment of Pathophysiology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, ChinaDepartment of Obstetrics and Gynecology, First Affiliated Hospital of Wenzhou Medical College, Wenzhou, Zhejiang, ChinaExcessive oxidative stress, decreased antioxidant capacity, and enhanced cellular calcium levels are initial factors that cause endothelial cell (EC) hyperpermeability, which represents a crucial event in the pathogenesis of pre-eclampsia. Lipoxin A4 (LXA4) strongly attenuated lipopolysaccharide (LPS)-induced hyperpermeability through maintaining the normal expression of VE-cadherin and β-catenin. This effect was mainly mediated by a specific LXA4 receptor. LXA4 could also obviously inhibit LPS-induced elevation of the cellular calcium level and up-regulation of the transient receptor potential protein family C 1, an important calcium channel in ECs. At the same time, LXA4 strongly blocked LPS-triggered reactive oxidative species production, while it promoted the expression of the NF-E2 related factor 2 (Nrf2) protein. Our findings demonstrate that LXA4 could prevent the EC hyperpermeability induced by LPS in human umbilical vein endothelial cells (HUVECs), under which the possible mechanism is through Nrf2 as well as Ca2+-sensitive pathways.http://dx.doi.org/10.1100/tsw.2011.98 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Huayan Pang Pan Yi Ping Wu Zhuoya Liu Zhongjie Liu Jianming Gong Hua Hao Lei Cai Duyun Ye Yinping Huang |
spellingShingle |
Huayan Pang Pan Yi Ping Wu Zhuoya Liu Zhongjie Liu Jianming Gong Hua Hao Lei Cai Duyun Ye Yinping Huang Effect of Lipoxin A4 on Lipopolysaccharide-Induced Endothelial Hyperpermeability The Scientific World Journal |
author_facet |
Huayan Pang Pan Yi Ping Wu Zhuoya Liu Zhongjie Liu Jianming Gong Hua Hao Lei Cai Duyun Ye Yinping Huang |
author_sort |
Huayan Pang |
title |
Effect of Lipoxin A4 on Lipopolysaccharide-Induced Endothelial Hyperpermeability |
title_short |
Effect of Lipoxin A4 on Lipopolysaccharide-Induced Endothelial Hyperpermeability |
title_full |
Effect of Lipoxin A4 on Lipopolysaccharide-Induced Endothelial Hyperpermeability |
title_fullStr |
Effect of Lipoxin A4 on Lipopolysaccharide-Induced Endothelial Hyperpermeability |
title_full_unstemmed |
Effect of Lipoxin A4 on Lipopolysaccharide-Induced Endothelial Hyperpermeability |
title_sort |
effect of lipoxin a4 on lipopolysaccharide-induced endothelial hyperpermeability |
publisher |
Hindawi Limited |
series |
The Scientific World Journal |
issn |
1537-744X |
publishDate |
2011-01-01 |
description |
Excessive oxidative stress, decreased antioxidant capacity, and enhanced cellular calcium levels are initial factors that cause endothelial cell (EC) hyperpermeability, which represents a crucial event in the pathogenesis of pre-eclampsia. Lipoxin A4 (LXA4) strongly attenuated lipopolysaccharide (LPS)-induced hyperpermeability through maintaining the normal expression of VE-cadherin and β-catenin. This effect was mainly mediated by a specific LXA4 receptor. LXA4 could also obviously inhibit LPS-induced elevation of the cellular calcium level and up-regulation of the transient receptor potential protein family C 1, an important calcium channel in ECs. At the same time, LXA4 strongly blocked LPS-triggered reactive oxidative species production, while it promoted the expression of the NF-E2 related factor 2 (Nrf2) protein. Our findings demonstrate that LXA4 could prevent the EC hyperpermeability induced by LPS in human umbilical vein endothelial cells (HUVECs), under which the possible mechanism is through Nrf2 as well as Ca2+-sensitive pathways. |
url |
http://dx.doi.org/10.1100/tsw.2011.98 |
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