Luciferase reporter assays to monitor interferon signaling modulation by SARS-CoV-2 proteins

Luciferase reporter assays to monitor interferon signaling modulation by SARS-CoV-2 proteins

Summary: We present a protocol for analyzing the impact of SARS-CoV-2 proteins in interferon signaling using luciferase reporter assays. Here, the induction of defined promoters can be quantitatively assessed with high sensitivity and broad linear range. The results are similar to those obtained usi...

Full description

Bibliographic Details
Main Authors: Maximilian Hirschenberger, Manuel Hayn, Alexandre Laliberté, Lennart Koepke, Frank Kirchhoff, Konstantin Maria Johannes Sparrer
Format: Article
Language:English
Published: Elsevier 2021-12-01
Series:STAR Protocols
Subjects:
Cell-based assays
Immunology
Microbiology
Molecular biology
Gene expression
Molecular/chemical probes
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721004871

Internet

http://www.sciencedirect.com/science/article/pii/S2666166721004871

Similar Items